Pro-subtilis (Bacillus subtilis DE111®)
Pro-subtilis promotes regularity by improving occasional constipation or diarrhea, reduces intestinal disturbances like gas or bloating.
DE111® plays a role in the recovery process, ensuring athletes are well prepared for training, competition and optimal performance.
Pro-subtilis is proven to support healthy microbiome in pre-school children and reduce stomach upset and gastrointestinal discomfort in children aged 2-6.
Bacillus subtilis is emerging as a promising probiotic that boosts immune capacity.
Pro-subtilis aids in the rebalancing of your own microbiota by stimulating increase of your own gut Bifidobacterium species and by reducing the Firmicutes-to-Bacteroidetes ratio (bad-to-good bacteria ratio).
Bacillus subtilis optimizes nutrient absorption by enhancing the efficiency of the digestive process.
The strain Bacillus subtilis DE111® has a lot of talents, which is why it has been nominated for "Microbe of the Year 2023."
Its role in improving gut function and supporting the immune system has made it a standout candidate in this prestigious category.
Clinical testing shows that the daily intake of Bacillus subtilis within the range of 1 to 5 billion CFU is just enough for its beneficial functions.
Furthermore, Bacillus subtilis DE111® promotes the growth of your own gut microflora Bifidobacterium species at a daily intake of 5 billion CFU.
Certain vitamins and minerals may interact with probiotics, potentially compromising their stability and colonization.
To ensure the maximum safety and effectiveness of the Bacillus subtilis DE111® probiotic strain, we have chosen to present it as a monoculture probiotic product.
In fact, Bacillus subtilis DE111® multiplies in the small intestine without the need for any prebiotics. Additionally, it produces short-chain fatty acids (SCFAs), which act as prebiotics for other beneficial bacteria in the gut.
For example, DE111® is clinically proven to stimulate the growth of your own gut's Bifidobacterium longum.
Each Pro-subtilis capsule is assured to contain a minimum of 5 billion CFU of Bacillus subtilis DE111® at the expiration date.
Storage stability is assessed in the United States.
Bacillus subtilis DE111® is one of the few probiotic strains that does not require refrigeration.
Bacillus family microbes are capable of forming spores, enabling them to endure challenging environmental conditions – like high temperatures or resisting gastric acid.
All probiotic strains, with almost no exceptions, are moisture sensitive.
Pro-subtilis probiotic is packaged in a special container designed to protect it from humidity.
Be sure to close the package tightly after use!
A crucial requirement for all probiotic actions and effects is that the probiotic strain can grow in your intestine.
Unlike Lactobacillus and even more so Bifidobacterium strains, which require specific conditions, Bacillus subtilis can grow easily in the intestine.
B. subtilis DE111® concentration in the small intestinal tract of healthy individuals with an ileostomy stoma. Vegetative DE111® (●), DE111® spores (■), and placebo (▲). Values are average concentrations (n = 11) ± standard deviation.
Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract: A Randomized, Crossover, Double-Blind, and Placebo-Controlled Study.
Researchers have found that consumption of Deerland Probiotics & Enzymes’ probiotic strain Bacillus Subtilis DE111® can improve occasional constipation or diarrhoea in healthy individuals, according to a study recently published in the Journal of Probiotics & Health.
A clinical study evaluating the effect of DE111® on digestive health resulted in a significant influence on gut microbiota composition measured prior to and after capsule consumption (5 billion CFU).
Faecal samples showed an increase in the counts of B. subtilis and Bifidobacterium (good bacteria) and a slight decrease in levels of E. coli (bad bacteria).
It is important to note that no Bifidobacteria spp. were supplemented in the trial.
In another study involving 91 children aged 2 to 6, researchers found that daily consumption of the probiotic strain Bacillus subtilis DE111® positively modulated the gut microbiome profile without changing the overall microbiome equilibrium.
After four weeks of daily consumption of the probiotic DE111® at 1 billion CFU a significant decrease in the basal levels of several immune cell populations, including CD3+ T cells, cytotoxic T cells, and NKT cells was observed.
Moreover, the probiotic group showed an increased capacity for immune response in cells stimulated by an induced inflammation.
The results suggests that DE111® may modulate the immune system through action on both innate (rapid response) and adaptive (slow response) immune cell types.
After 8 weeks of daily probiotic consumption at 1 billion CFU, the researchers observed an increase in diversity at the phylum level, suggesting an expanded functional diversity of the microbiome compared to that of the children in the placebo group.
Specifically, there were 9 differentially abundant taxa at the genus level found in the probiotic group, six (of the phylum Bacteroidetes) grew in abundance while three (of the phylum firmicutes) were reduced. The six taxa of Bacteroidetes that flourished are involved in immune regulation and reduction of inflammation.
This modulation of the firmicutes/Bacteroidetes ratio of the microbiome in children taking B. subtilis DE111® is a positive indication for healthy gut function.
Using a combination of in-vitro functional assays and genome analysis, researchers found that DE111® demonstrated the ability to survive harsh conditions such as those found in the GI tract, as well as pasteurization.
It also produces short-chain fatty acids, which support healthy lower GI function, as well as an increase in enzymatic activity (such as proteases, peptidases and esterases) and carbohydrate metabolism, pointing to its ability to promote a more efficient digestive process.
Additionally, researchers observed the strain’s ability to outcompete several types of unwanted bacteria associated with urinary, skin and intestinal flora imbalances.
In a study involving male collegiate athletes during offseason training, researchers found that DE111 (1 billion CFU), in conjunction with adequate post-workout nutrition, can promote tissue recovery and reduce likelihood of injury.
The results of the study showed that compared to the placebo, the probiotic DE111® produced a statistically significant reduction of tumor necrosis factor alpha (TNA-α).
In a study involving female collegiate athletes during offseason training, researchers found that DE111® (1 billion CFU), in conjunction with adequate post-workout nutrition, can improve body composition and indices of athletic performance.
The results of the study showed that compared to placebo, the probiotic DE111® produced statistically significant improvements in the reduction of body fat percentage and a strong trend indicating improved performance of the deadlift exercise.
